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SPI Supplies
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Fluka Chemical
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Philips Healthcare
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SERVA Electrophoresis
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Ted Pella
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Fisher Scientific
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Katayama Chemical Inc
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Agar Scientific
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Philips Healthcare
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Merck KGaA
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Image Search Results
Journal: Nature methods
Article Title: Nanoscale 3D cellular imaging by axial scanning transmission electron tomography
doi: 10.1038/nmeth.1367
Figure Lengend Snippet: Scanning transmission electron microscopy using axial detection. ( a ) Schematic diagram depicting the influence of STEM detector configuration on the spatial resolution of specimen features situated towards the bottom of thick sections. Light blue arrow represents a STEM probe incident on a thick specimen. Dark blue lines represent electrons that arrive at the bottom of the specimen with little displacement from the center of the probe, whereas red dashed lines represent electrons that arrive at the bottom with large displacements from the center. Black dotted line indicates the central axis through the specimen defined by the center of the incident STEM probe. Inset: detail of bottom region of specimen showing electron trajectories resulting in small displacements (blue lines) and large displacements (red lines) relative to the center of the probe. ( b–f ) Projection images from a 1 μm-thick section of P. falciparum -infected erythrocyte. BF STEM revealing particles both on top surface (arrows) and bottom surface (arrowheads) ( b ), HAADF STEM with contrast inverted showing only particles on top surface (arrows) ( c ), conventional TEM ( d ), and energy-filtered TEM at the most-probable energy-loss ( e , f ). Electron dose was 10 3 e/nm 2 in all images except in f , for which the dose was 10 4 e/nm 2 . Scale bar, 100 nm.
Article Snippet: Next, we stained sections containing infected
Techniques: Transmission Assay, Electron Microscopy, Infection